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clinmed/2001100002v1 (October 24, 2001)
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ANNEXIN V AND COMET ASSAY DETECTION OF VIOLACEIN-INDUCED APOPTOSIS

Pedro H.P.A. Schildknecht, and Benedicto C. Vidal

The apoptosis triggered by violacein in V79 cells was assessed using annexin V/propidium iodide labeling and the Comet assay. No necrosis was detected by propidium iodide staining. Apoptotic nuclei were labeled by annexin V, and the results agreed with those with the Comet assay in weak alkaline solution. Characteristic apoptotic nuclear phenotypes were obtained from violacein-treated cells just lysis step. The use of annexin V/propidium iodide staining together with the Comet assay provided a fast, reliable means of characterizing cell death. Chromatin fragmentation and the externalization of phosphatidylserine were visualized by annexin V/propidium iodide staining after less than 48 h of exposure to violacein.





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