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clinmed/2002040002v1 (April 24, 2002)
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Detection of differentially expressed genes in LPS treated human renal tubular epithelial cells using c DNA arrays

HIRENDRA BANERJEE

ABSTRACT This study reports the use of gene array technology for the identification of differentially expressed genes in Lipopolysaccharide (LPS) treated human renal tubular epithelial cells. The tubular epithelial response to bacterial infection has not been well studied at a molecular level. In order to study differential Gene expression in response to LPS stimulation, cultured tubular epithelial cells were treated with 1mg/ml LPS for 24 hours. RNA was isolated from the treated and control cells and cDNA synthesized by Reverse Transriptase reaction. Radio labeled cDNA was hybridized to a cDNA human expression array with several known genes.The results showed upregulation of several genes in response to LPS including ubiquitin, zinc finger protein 91 (ZNF92), G2 mitotic specific cyclin (CCNA) etc being the novel and the most notable ones. This study illustrates the enormous potential of gene array technologies for understanding the molecular mechanisms involved in kidney diseases caused by bacterial infection.





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